Orbitally shaken bioreactors-viscosity effects on flow characteristics
نویسندگان
چکیده
منابع مشابه
Hydrodynamic stress in orbitally shaken bioreactors
Materials and methods CHO-DG44 cells were cultivated in suspension in 1-L bottles as described in [1]. To determine conditions under which the shear stress was harmful for the cells, the bottles were orbitally shaken on an ES-X platform (Kühner AG, Birsfelden, Switzerland) at agitation rates from 150 to 200 rpm for 24 h. Control cultures were run in parallel with agitation at 110 rpm. The veloc...
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Rationale and significance. Recombinant therapeutic proteins are frequently produced in mammalian cells cultivated in suspension in large-scale stirred tank bioreactors. There are three major drawbacks to stirred-tank bioreactors. First, they are expensive to purchase and maintain, limiting their availability to some potential users. Second, they have not been adapted to volumetric scales less ...
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Background Orbitally shaken flasks are commonly used at an early stage of bioprocess development with mammalian cells. In contrast to large-scale stirred-tank bioreactors, shaken flasks are usually operated in probe-independent bioprocesses, i.e. without strictly controlling the pH or dissolved oxygen concentration (DO). As a consequence, gas transfer issues are thought to limit the effectivene...
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Mammalian cell cultures have become a major topic of research in the biopharmaceutical industry. This kind of cells require specific conditions to grow. In this thesis, we study the hydrodynamics of orbitally shaken reactors (OSR), a recently introduced kind of bioreactors for mammalian cell cultures that represents a simple to operate and cheap alternative to commonly used reactors such as sti...
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In design and analysis, standard references assume the pedestrian flow as unidirectional. In reality however, pedestrian flow is usually bi-directional. The main question pursued in this paper is that whether the main characteristics of pedestrian flow the same under uni- and bi-directional conditions. In order to achieve this goal, effect of bi-directional stream is inv...
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ژورنال
عنوان ژورنال: AIChE Journal
سال: 2014
ISSN: 0001-1541
DOI: 10.1002/aic.14608